indirect immunofluorescence Synonyms

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indirect immunofluorescence Meaning

Wordnet

indirect immunofluorescence (n)

a method of using fluorescence microscopy to detect the presence of an antigen indirectly

FAQs About the word indirect immunofluorescence

a method of using fluorescence microscopy to detect the presence of an antigen indirectly

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No antonyms found.

Indirect immunofluorescence (IIF) is employed in immunology to detect antigens by visualizing their fluorescently labeled antibodies.

IIF utilizes secondary antibodies that are conjugated to fluorescent dyes to amplify the signal.

The technique involves incubating the sample with primary antibodies specific to the target antigen.

Unbound primary antibodies are washed away, followed by the addition of fluorescently labeled secondary antibodies.