indirect immunofluorescence Sentence Examples
- Indirect immunofluorescence (IIF) is employed in immunology to detect antigens by visualizing their fluorescently labeled antibodies.
- IIF utilizes secondary antibodies that are conjugated to fluorescent dyes to amplify the signal.
- The technique involves incubating the sample with primary antibodies specific to the target antigen.
- Unbound primary antibodies are washed away, followed by the addition of fluorescently labeled secondary antibodies.
- IIF allows for the visualization of antigens in tissue sections or on cell surfaces.
- It is widely used in diagnostic pathology for the identification of infectious agents and autoimmune diseases.
- IIF can also be employed in research to study protein localization and interactions.
- The sensitivity of IIF can be enhanced by using high-affinity antibodies and bright fluorescent dyes.
- IIF is less expensive than other immunofluorescence techniques and can be easily performed in a laboratory setting.
- The results of IIF are typically analyzed using a fluorescence microscope to detect the fluorescently labeled antigens.
indirect immunofluorescence Meaning
Wordnet
indirect immunofluorescence (n)
a method of using fluorescence microscopy to detect the presence of an antigen indirectly
Synonyms & Antonyms of indirect immunofluorescence
No Synonyms and anytonyms found
FAQs About the word indirect immunofluorescence
a method of using fluorescence microscopy to detect the presence of an antigen indirectly
No synonyms found.
No antonyms found.
Indirect immunofluorescence (IIF) is employed in immunology to detect antigens by visualizing their fluorescently labeled antibodies.
IIF utilizes secondary antibodies that are conjugated to fluorescent dyes to amplify the signal.
The technique involves incubating the sample with primary antibodies specific to the target antigen.
Unbound primary antibodies are washed away, followed by the addition of fluorescently labeled secondary antibodies.
