fluorescein isothiocyanate Synonyms
No Synonyms and anytonyms found
fluorescein isothiocyanate Meaning
Wordnet
fluorescein isothiocyanate (n)
a fluorochrome commonly conjugated with antibodies for use in indirect immunofluorescence
fluorescein isothiocyanate Sentence Examples
- Fluorescein isothiocyanate (FITC) is a highly fluorescent molecule used for labeling proteins and other biological molecules.
- FITC conjugates can be visualized using fluorescence microscopy, making them valuable tools for studying protein localization and function.
- FITC was first synthesized in 1948 and has since become one of the most widely used fluorescent dyes in biology.
- FITC emits green fluorescence upon excitation with blue light, making it ideal for use with fluorescence filters in microscopy.
- The isothiocyanate group of FITC reacts with the primary amine groups of proteins, forming a covalent bond.
- FITC-labeled antibodies are commonly used in immunohistochemistry and flow cytometry to detect specific proteins in cells and tissues.
- FITC-labeled lectins are used to study carbohydrate-binding interactions and cell surface receptors.
- FITC can be used to label nucleic acids for fluorescent in situ hybridization (FISH).
- FITC is a relatively stable molecule that is resistant to photobleaching and enzymatic degradation.
- Despite its widespread use, FITC can sometimes cause nonspecific labeling and background fluorescence, which should be considered when using it in assays.
FAQs About the word fluorescein isothiocyanate
a fluorochrome commonly conjugated with antibodies for use in indirect immunofluorescence
No synonyms found.
No antonyms found.
Fluorescein isothiocyanate (FITC) is a highly fluorescent molecule used for labeling proteins and other biological molecules.
FITC conjugates can be visualized using fluorescence microscopy, making them valuable tools for studying protein localization and function.
FITC was first synthesized in 1948 and has since become one of the most widely used fluorescent dyes in biology.
FITC emits green fluorescence upon excitation with blue light, making it ideal for use with fluorescence filters in microscopy.
